Workshop Contacts
NSLS User Administration
Gretchen Cisco
Phone: 631.344.4703
Email: gcisco@bnl.gov

Vivian Stojanoff
Phone: 631.344.8375
Email: stojanof@bnl.gov

Crystallization: Focus on optimization techniques for soluble and membrane proteins

Agenda


Sunday, June 5th
15:30 - 18:30 Registration in the User Office
Monday, June 6th
08:00 - 09:15Registration in the User Office and Coffee
09:15 - 09:20Welcome and Coffee
09:20 - 10:00Neer Asherie: Understanding Protein Phase Behavior
10:00 - 10:40Pat Loll: Membrane Proteins and Detergents
10:40 - 11:00Break
11:00 - 11:40Marie-Claude Marchand: The Vapor Diffusion Method and Optimization
11:40 - 12:15Short presentations by those participants who brought their own samples for crystallization trials
12:15 - 12:30Chi-Chang Kao: Introduction to the NSLS
12:30 - 13:45Lunch Check your groups
13:45 - 15:45Practical Session I
15:45 - 16:00Break
16:00 - 18:00Practical Session II
18:00 - 19:00Naomi Chayen: Non-standard Crystallization Techniques
19:00Dinner
Tuesday, June 7th
08:30 - 09:00Coffee
09:00 - 09:40Petra Fromme: The Role of the Phase Diagram in the Crystallization of PSI and PSII?
09:40 - 10:20Peter Nollert: Micro Crystallization using the Lipidic Cubic Phase Methodology
10:20 - 10:40Break
10:40 - 12:40Practical Session III
12:40 - 13:50Lunch
13:50 - 15:50Practical Session IV
15:50 - 16:10Break
16:10 - 18:10Practical Session V
19:00Dinner
Wednesday, June 8th
08:30 - 09:00Coffee
09:00 - 11:00Practical Session VI
11:00 - 11:15Break
11:15 - 13:15Practical Session VII
13:15 - 14:30Lunch
14:30 - 16:30Practical Session VIII
16:30 - 17:00Close-out and Coffee
Optional
14:30 - 18:00Check Crystal Diffraction. Practical on Cryo protection and automounter at X6A the beam line. This could continue through the morning of the Thursday June 9th
Practicals
1. Purification; JanMeet Anant and Debbie Cohen, GE Healthcare
2. Detergent Effects: Pat Loll, Drexel University
3. Light Scattering: Trevor Harvard (Precision Detectors) Neer Asherie (Yeshiva University)
4. Vapor Diffusion, how to improve your crystal: Marie Claude Marchand, Caroline Charky (Nextal Biotechnology)
5. Portfolio of optimization techniques: Naomi Chayen
6. The Dialysis Method, PSI and PSII: Petra Fromme (Arizona State University)
7. Crystallization in the Lipid Cubic Phase: Peter Nollert (deCode Genetics)
8. Screening for High Throughput: Chris Gawroski, Chris Dankulick (Fluidigm)