Workshop Contacts
NSLS User Administration
Gretchen Cisco
Phone: 631.344.4703
Email: gcisco@bnl.gov

Vivian Stojanoff
Phone: 631.344.8375
Email: stojanof@bnl.gov

Crystallization: Focus on Optimization and High Throughput Techniques

Agenda

Monday, June 12th
08:30 - 12:00Registration - NSLS User Office
12:00 - 13:00Lunch on your own (BNL Cafeteria)
13:00 - 13:15Welcome and Coffee - NSLS Seminar Room
13:15 - 13:30Chi-Chang Kao (NSLS)
Introduction to the NSLS
13:30 - 14:10Neer Asherie (Yeshiva University)
Understanding Protein Phase Behavior
14:10 - 14:50Marie-Claude Marchand (Qiagen, Inc.)
The Vapor Diffusion Method and Optimization
14:50 - 15:10Break and Group Photo - NSLS Seminar Room
15:10 - 15:50Gwen Nneji (Imperial College London)
Non-standard Crystallization Techniques
15:50 - 16:30Abel Moreno (Universidad Nacional Autonoma de Mexico)
Gels and Fields: What Can They Say About the Phase Diagram?
16:30 - 16:40Break - NSLS Seminar Room
16:40 - 17:20Pat Loll
Membrane Proteins and Detergents
17:20 - 18:00Peter Nollert (Emerald BioSystems)
Micro Crystallization using the Lipidic Cubic Phase Methodology
18:00 - 18:40Ingo Grotjohann (Arizona State University)
The Role of the Phase Diagram in the Crystallization of PSI and PSII
18:50Dinner, Car Pool
Curry Club
766 Rt. 25A in E. Setauket, Long Island 11733 (corner of Nichols Rd. & 25A) - 631.751.4845
Tuesday, June 13th
08:15 - 09:00Breakfast - NSLS Library
08:30 - 09:30Discussion with those who brought proteins. This is the time to share your problems.
09:00 - 12:15Practical Session I
10:45 - 11:00Coffee - NSLS Library
12:15 - 13:15Lunch - NSLS Library
13:15 - 16:00Practical Session II
16:00 - 16:30Coffee - NSLS Library
16:30 - 18:00NSLS Tour
16:30 - 18:00Peek-a-Boo Session
18:00Dinner (BBQ weather permitting)
Discussion
Wednesday, June 14th
08:15 - 08:45Breakfast - NSLS Library
08:50 - 9:20Craig Sterling (Emerald BioSystems)
Enabling Technologies in High-Throughput Crystallography
09:30 - 12:00Practical Session III
10:45 - 11:00Coffee - NSLS Library
12:15 - 13:15Lunch - NSLS Library
13:15 - 16:00Practical Session IV
16:00 - 16:30Coffee - NSLS Library
16:30 - 17:00Tom Burke (GE Healthcare)
Protein Purification...Every Last Drop
17:00 - 18:30Peek-a-Boo Session
18:30Dinner
Discussion
Thursday, June 15th
08:15 - 08:45Breakfast - NSLS Library
08:50 - 09:20Patrice Goorec (Structural Genomics Consortium)
Application of SGC Oxford protein crystallization platform
09:30 - 12:00Practical Session V
10:45 - 11:00Coffee - NSLS Library
12:15 - 13:15Lunch - NSLS Library
13:15 - 14:00Close Out
Optional
14:00 - 19:00Check Crystal Diffraction. Practical on Cryo protection and automounter at X6A
Practicals
1. Improved Purification Strategies: difficult to purify or low expressed proteins: Troy Burke, Martha Foley (GE Healthcare)
2. Cell free expression system and mass spectroscopy in high throughput proteomics: Marcia Amstrong (Qiagen)
3. Vapor Diffusion, how to improve your crystal: Marie Claude Marchand (Qiagen)
4. Portfolio of optimization techniques: Gwen Nneji (Imperial College London)
5. Gels and Fields: optimizing the phase diagram of soluble proteins: Abel Moreno (Universidad Nacional Autonoma de Mexico)
6. Light Scattering: how best to use it to improve your crystallization conditions: Trevor Harvard (Precision Detectors), Neer Asherie (Yeshiva University)
7. Detergent Effects: Pat Loll (Drexel University)
8. Automated Screen Formulation for (Membrane) Protein Crystallization: Craig Sterling, (Emerald BioSystems)
9. Crystallization in the Lipid Cubic Phase: Peter Nollert (Emerald BioSystems)
10. The Dialysis Method PSI: Ingo Grotjohann (Arizona State University)
11. Screening for High Throughput: Chris Gawroski, Chris Dankulick (Fluidigm)