Workshop Contacts
NSLS User Administration
Gretchen Cisco
Phone: 631.344.4703
Email: gcisco@bnl.gov

Vivian Stojanoff
Phone: 631.344.8375
Email: stojanof@bnl.gov

Crystallization: Focus on Membrane Proteins

Agenda

Tuesday, June 19
8:30 - 12:00Registration in the User Office
12:00 - 1:00Lunch on your own (BNL Cafeteria)
1:00 - 1:30Welcome
Coffee - NSLS Seminar Room
1:30 - 2:10Marcia Armstrong (Qiagen)
"Vapor Diffusion and Optimization with a focus on Membrane Protein"
2:10 - 2:50Gwen Nneji (Birkbek University of London)
"Modified Methods for Crystallization"
2:50 - 3:00Break - NSLS Seminar Room
3:00 - 3:40Patrick Loll (Drexel University)
"Membrane proteins and detergents"
3:40 - 4:20Peter Nollert (Emerald BioSystems)
"The Lipidic Cubic Phase as a Matrix for Membrane Protein Crystallization"
4:20 - 4:30Break - NSLS Seminar Room
4:30 - 5:10Michael Wiener (University of Virginia)
"A Compendium of Membrane Protein Crystallization"
5:10 - 7:00Discussion Group Set Up
7:00Dinner
Wednesday, June 20
8:30 - 9:00Breakfast - NSLS Library
9:00 - 9:40Abel Moreno (UNAM)
"The use of gels and magnetic fields in membrane protein crystallization"
9:40 - 10:00Coffee - NSLS Library
10:00 - 12:30Practical I - Session I
12:30 - 1:30Lunch - NSLS Library
1:30 - 2:10Rustem Ismagilov (University of Chicago)
"Crystallization of membrane proteins in nanoliter volumes using plug-based microfluidics"
2:10 - 2:30Coffee - NSLS Library
2:30 - 5:00Practical I - Session II
5:00 - 6:30Peek-a-Boo Session I
6:30 - 7:00Discussion
7:00Dinner
Thursday, June 21
8:30 - 9:00Breakfast - NSLS Library
9:00 - 9:40Ingo Grotjohann (University of Arizona)
"The PSI phase diagram and its role in the crystallization"
9:40 - 10:00Coffee - NSLS Library
10:00 - 12:30Practical I - Session III
12:30 - 1:30Lunch - NSLS Library
1:30 - 2:10Neer Asherie (Yeshiva University)
"Understanding protein phase behavior"
2:10 - 2:30Coffee - NSLS Library
2:30 - 5:00Practical I - Session IV
5:00 - 6:30Peek-a-Boo Session II
6:30 - 7:00Discussion
7:00Dinner
Friday, June 22
8:30 - 9:00Breakfast - NSLS Library
9:00 - 9:40Troy Burke (GE Healthcare)
"Improved Purification Strategies"
9:40 - 10:00Coffee - NSLS Library
10:00 - 12:30Practical I - Session V
12:30 - 1:30Lunch - NSLS Library
1:30 - 2:10Bert van den Berg (University of Massachussets)
"Practical aspects of membrane protein crystallization"
2:10 - 2:30Coffee - NSLS Library
2:30 - 5:00Practical I - Session VI
5:00 - 7:00Practical II - Session I
7:00Dinner
Saturday, June 23
8:30 - 9:00Breakfast - NSLS Library
9:00 - 12:30Practical II - Session II
12:30 - 1:30Lunch - NSLS Library

Practical I

  1. Purification strategies for membrane protein crystallography - Troy Burke (GE Healthcare)
  2. Practical crystallization screening using vapor diffusion - Michael Wienner (University of Virginia)
  3. Modified crystallization methods - Gwen Nneji (Birkbeck University of London)
  4. The use of gels and magnetic fields in membrane protein crystallization - Abel Moreno (Universidad Nacional Autonoma de Mexico)
  5. Dynamic Light Scattering - Trevor Harvard (Precision Detectors), Neer Asherie (Yeshiva University)
  6. Crystallization in the Lipid Cubic Phase: Peter Nollert (Emerald BioSystems)
  7. The dialysis method applied to PSI - Ingo Grotjohann (Arizona State University)
  8. Plug-based microfluidic crystallization experiments - Liang Li (University of Chicago), Mark Elliot (Emerald BioSystems)

Practical II

  1. Cryoprotection and X-ray diffraction
  2. Seetharamam Jayaramam (Columbia University, NYSBC), X6A Team

Peekaboo

  1. Cell free expression system - Marcia Amstrong (Qiagen)
  2. Working with detergents - Pat Loll (Drexel University)