Workshop Contact

Ann Emrick
Phone: 631.344.5756
Email: BioCD@bnl.gov

BioCD-2008: Short Course in Circular Dichroism Spectroscopy of Proteins and other Biomolecules

Agenda


Monday, June 23

8:00 - 11:30Check In at User Administration (Building 400)
12:15 - 1:30Lunch*
1:30 - 2:30Introduction to BNL, the NSLS and the CD Workshop and Principles of CD Spectroscopy: John Sutherland
2:30 - 3:30CD of Proteins, an Overview: Bonnie Wallace
3:30 - 4:00Coffee/Tea Break*
4:00 - 5:00Determination of Protein Secondary Structure and Practical Considerations: Bonnie Wallace
5:00 - 6:00Planning of Laboratory Sessions: All

Tuesday, June 24

9:00 - 10:00CD of Nucleic Acids: Alison Rodger
10:00 - 11:00CD Bioinformatics: Websites, Dichroweb and CDtools: Bonnie Wallace
11:00 - 11:15Coffee/Tea Break
11:15 - 12:15Demonstration of Dichroweb, web based software for analysis of protein secondary structure: Lee Whitmore
Demonstration of CDtools, personal computer based software for analysis of protein secondary structure: Bonnie Wallace
12:15 - 1:30Lunch
1:15Group Photo
1:30 - 3:30Laboratory I CD Data Acquisition using conventional and synchrotron source instruments (Student groups will rotate)
3:30 - 4:00Coffee/Tea Break
4:00 - 6:00Laboratory II Data Acquisition using conventional and synchrotron source instruments (Student groups will rotate)

Wednesday, June 25

9:00 - 10:00CD of Membrane Proteins: Bonnie Wallace
10:00 - 11:00Data Bases for CD of Globular and Membrane Bound Proteins: Bob Janes
11:00 - 11:15Coffee/Tea Break
11:15 - 12:15Linear Dichroism: Alison Rodger
12:15 - 1:30Lunch
1:30 - 3:30Laboratory III CD Data Acquisition using conventional and synchrotron source instruments (Student groups will rotate)
3:30 - 4:00Coffee/Tea Break
4:00 - 6:00Laboratory IV CD Data Acquisition using conventional and synchrotron source instruments (Student groups will rotate)

Thursday, June 26

9:00 - 10:00Measurement of CD, Magnetic CD, Fluorescence Detected CD/MCD, LD and Fluorescence Polarization Anisotropy using the Photoelastic Modulator: John Sutherland
10:00 - 11:00Vibrational Spectroscopy and Imaging of Biological Systems Using Synchrotron Radiation: Lisa Miller
11:00 - 11:15Coffee/Tea Break
11:15 - 12:15Binding of Drugs, Carcinogens and other Ligands to Proteins and Nucleic Acids: Alison Rodger
12:15 - 1:30Lunch
1:30 - 3:30Laboratory V Data Analysis (Student groups will rotate)
Dichroweb: Lee Whitmore
Websites and CDtools: Janes/Wallace
3:30 - 4:00Coffee/Tea Break
4:00 - 6:00Laboratory VI Data Analysis (Student groups will rotate)
Dichroweb: Lee Whitmore
Websites and CDtools: Janes/Wallace

Friday, June 27

9:00 - 10:00Time Resolved (Stopped Flow) CD, Magnetic CD and the extension of CD spectroscopy into the infrared and X-ray spectral regions: John Sutherland
10:00 - 11:00Applications of CD to Structural and Functional Genomics: Bob Janes
11:00 - 11:15Coffee/Tea Break
11:15 - 12:30Student presentations of experimental results and discussion of future directions in CD spectroscopy
12:45 - 1:30Lunch
1:30 - 2:30Special Problems (by arrangement)

 *All lectures and laboratory exercises plus lunch and coffee/tea breaks will be at the National Synchrotron Light Source (Building 725).

Breakfast will be available on site at the BNL Cafeteria (Located in Berkner Hall, Bldg. 488) beginning at 7:30 AM).

Dinner will be available on site at the Brookhaven Center (located in Bldg. 30 across from Police Headquarters) from 5 to 8 PM, with limited menu service continuing until 9 PM. The Center closes at 10:00 PM.

The cost of breakfasts and dinners are not included in the registration.