2002 NSLS Users' Meeting

Practical Aspects of Membrane Protein Crystallography: From Overexpression to Crystallization

Chemistry Dept. Bldg. 555 - Hamilton Room

Monday, May 20, 2002

Organizer: Larry Shapiro, Columbia University, 212-342-6029, and Filippo Mancia, Columbia University

Integral membrane proteins perform some of the most important functions of living cells, yet understanding their molecular mechanisms through structural studies presents unique challenges. The aim of this workshop is to discuss the technical aspects (“nuts-and-bolts”) of overexpressing, purifying, and crystallizing membrane proteins for structural studies by x-ray crystallography

9:00 a.m.	Welcome and Introduction
9:15 a.m.	Susan Buchanan, NIH, "Expression, Purification and Crystallization of Bacterial Outer Membrane Proteins"
9:45 a.m.	Da Neng Wang, NYU, "Expression and Characterization of Bacterial Membrane Transporters"
10:15 a.m.	Coffee Break
10:45 a.m.	Martine Cadene, Rockefeller University, "Key Mass Spectrometric Tools for the Crystallography of Membrane Proteins"
11:15 a.m.	Miro Venturi, NIH, "Crystallization of Membrane Proteins with Monoclonal Antibody Fragments"
11:45 a.m.	Lunch Break at workshop location
1:15 p.m.	Reinhard Grisshammer, NIH, "Expression and Purification of G-Protein-Coupled Receptors for Structure Determination"
1:45 p.m.	Barry Springer, 3D Pharmaceuticals, "Expression and Purification and Stabilization of G-Protein Coupled Receptors for X-ray Crystallography"
2:15 p.m.	Coffee Break
2:30 p.m.	Ehud Landau, University of Texas, "Molecular Mechanism for the Cubo Crystallization of Membrane Proteins"
3:00 p.m.	Dax Fu, BNL, "Stabilization of Membrane Proteins for Crystallization"
3:30 p.m.	Pat Loll, Drexel, "Rational Approaches to Membrane Protein Crystallization: Focusing on Detergent Effects"
4:00 p.m.	Closing Remarks