|
|
Workshop #8 at the NSLS Users' Meeting
Crystallization, Membrane Proteins
Registration Closed
Location: Bldg. 725, NSLS Seminar Rm
Date: May 19-20, 2004
Organizers:
Naomi Chayen, Imperial College of Science, Technology & Medicine (n.chayen@ic.ac.uk)
Vivian Stojanoff, BNL (stojanof@bnl.gov)
Description: The workshop will cover different
crystallization methods from the most standard vapor diffusion
methods to more advanced techniques. A short introduction to the
crystallization process will be followed by a series of hands-on
experiments. Participants will be able to crystallize there own PSI
and PSII proteins and use the lipid cubic phase to crystallize their
own samples. The goal is for all participants to be able to try out
the different methods under discussion.
Two topics will be covered
in five practicals:
- Soluble Proteins
- Marie Claude Marchand (Nextal) - "The Vapor Diffusion
Method"
- Ana Belen Moradela Merlo (University of Granada) - "Protein
Crystallization by the Counter Diffusion Method"
- Membrane Proteins
- Petra Fromme (Arizona State University) - "Crystallization of PSI and PSII,
a phase diagram problem"
- Peter Nollert (deCODE Genetics, Inc) - "Crystallization
in Lipid Cubic Phase"
- Naomi Chayen - "Screening and optimization techniques
using oils, seeding and other means"
The workshop is limited to 20 participants due to its hands-on
character. It is on a first come first serve basis. Participants are
welcome to bring their own samples.
All participants of this workshop need a VALID BNL GUEST NUMBER
since the workshop will take place on the experimental floor. Please
make sure to register for both: the workshop and to obtain a valid
BNL Guest appointment. Due to increased site security foreign
nationals need to allow at least 30 days for security clearance.
https://fsd84.bis.bnl.gov/guest/
For further information please contact the organizers
Schedule:
| May 19 |
| 8:00 a.m. |
Coffee |
NSLS Seminar Room |
| 8:30 a.m. |
Welcome and Registration |
NSLS Seminar Room |
| 9:30 a.m. |
Vivian Stojanoff (NSLS) Crystallization – An Overview |
NSLS Seminar Room |
| 10:00 a.m. |
Coffee Break |
NSLS Seminar Room |
| 10:15 a.m. |
Naomi Chayen (Imperial College of London) Tackling the bottleneck of protein crystallisation: Practical techniques with a difference |
NSLS Seminar Room |
| 10:55 a.m. |
Peter Nollert (deCODE Genetics) Miniaturization of the cubic phase |
NSLS Seminar Room |
| 11:35 a.m. |
Petra Fromme (Arizona State University) Ovecoming the crystallisation problems of PSI and PSII |
NSLS Seminar Room |
| 12:10 p.m. |
Lunch |
NSLS Library |
| 1:15 p.m. |
Practicals 1 Marie Claude Marchand, Ana Belen Moradela Merlo, Petra Fromme, Peter Nollert, Naomi Chayen |
|
| 2:45 p.m. |
Break |
NSLS Conf Room C |
| 3:00 p.m. |
Practicals 2 Marie Claude Marchand, Ana Belen Moradela Merlo, Petra Fromme, Peter Nollert, Naomi Chayen |
|
| 4:30 p.m. |
Break |
NSLS Conf Room C |
| 4:45 p.m. |
Practicals 3 Marie Claude Marchand, Ana Belen Moradela Merlo, Petra Fromme, Peter Nollert, Naomi Chayen |
|
| May 20 |
| 8:00 a.m. |
Coffee |
NSLS Conf Room C |
| 8:30 a.m. |
TBA |
|
| 9:00 a.m. |
Practicals 4 Marie Claude Marchand, Ana Belen Moradela Merlo, Petra Fromme, Peter Nollert, Naomi Chayen |
|
| 10:30 a.m. |
Coffee Break |
NSLS Conf Room C |
| 10:45 a.m. |
Practicals 5 Marie Claude Marchand, Ana Belen Moradela Merlo, Petra Fromme, Peter Nollert, Naomi Chayen |
|
| 12:15 p.m. |
Lunch |
NSLS Library |
| 1:00 p.m. |
Practicals revisited Marie Claude Marchand, Ana Belen Moradela Merlo, Petra Fromme, Peter Nollert, Naomi Chayen |
|
| 2:30 p.m. |
Closing discussions |
NSLS Seminar Room |
| 3:00 p.m. |
Practicals revisited Marie Claude Marchand, Ana Belen Moradela Merlo, Petra Fromme, Peter Nollert, Naomi Chayen |
|
|